Table 3.

In vitro activities of TBG-MINO and comparative antibiotics against gram-negative isolates

Organism (no. of isolates)AntibioticMIC (μg/ml)
Range50%90%
Escherichia coli(minocycline MIC, ≥1 μg/ml (32)TBG-MINO0.25–10.50.5
DMG-MINO0.25–40.54
DMG-DMDOT0.25–414
Minocycline1–32816
Tetracycline2–>32>32>32
Ciprofloxacin0.008–320.0080.015
Imipenem0.06–0.250.120.12
Ceftazidime0.06–10.120.25
Escherichia coli (minocycline MIC, ≤0.5 μg/ml) (14)TBG-MINO0.25–0.50.50.5
DMG-MINO0.25–0.50.250.5
DMG-DMDOT0.50.50.5
Minocycline0.25–0.50.50.5
Tetracycline1–212
Ciprofloxacin≤0.004–0.25≤0.0040.03
Imipenem0.06–0.120.120.12
Ceftazidime0.06–0.250.120.12
Shigella spp. (26)TBG-MINO0.12–0.50.250.5
DMG-MINO0.12–10.250.5
DMG-DMDOT0.12–10.50.5
Minocycline0.25–1624
Tetracycline1–>32>32>32
Ciprofloxacin≤0.004–0.015≤0.0040.008
Imipenem0.06–0.50.120.25
Ceftazidime0.06–0.120.120.12
Klebsiella pneumoniae(10)TBG-MINO0.5–212
DMG-MINO0.5–111
DMG-DMDOT0.5–111
Minocycline1–424
Tetracycline1–422
Ciprofloxacin0.008–0.030.030.03
Imipenem0.12–0.50.120.25
Ceftazidime0.06–0.50.120.12
Klebsiella oxytoca(10)TBG-MINO111
DMG-MINO111
DMG-DMDOT111
Minocycline2–822
Tetracycline2–>3222
Ciprofloxacin0.008–0.030.0150.015
Imipenem0.12–0.250.120.25
Ceftazidime0.06–0.50.120.12
Citrobacter freundii(10)TBG-MINO0.5–812
DMG-MINO0.5–812
DMG-DMDOT1–811
Minocycline1–3244
Tetracycline1–1622
Ciprofloxacin≤0.004–160.0150.12
Imipenem0.25–20.51
Ceftazidime0.12–>320.58
Citrobacter diversus(10)TBG-MINO0.5–211
DMG-MINO0.5–211
DMG-DMDOT1–211
Minocycline1–424
Tetracycline2–824
Ciprofloxacin≤0.004–0.060.0080.06
Imipenem0.06–120.060.12
Ceftazidime0.12–0.50.120.5
Salmonella spp. (14)TBG-MINO0.25–211
DMG-MINO0.5–40.51
DMG-DMDOT0.5–40.51
Minocycline0.5–32216
Tetracycline1–>322>32
Ciprofloxacin≤0.004–0.030.0150.03
Imipenem0.06–0.250.120.25
Ceftazidime0.12–0.50.250.5
Serratia marcescens(10)TBG-MINO4–844
DMG-MINO4–848
DMG-DMDOT4–844
Minocycline4–888
Tetracycline8–>3232>32
Ciprofloxacin0.008–20.120.25
Imipenem0.25–20.52
Ceftazidime0.12–10.250.5
Enterobacter cloacae(10)TBG-MINO1–212
DMG-MINO1–212
DMG-DMDOT1–212
Minocycline2–444
Tetracycline2–444
Ciprofloxacin≤0.004–0.060.030.03
Imipenem0.250.250.25
Ceftazidime0.12–>320.25>32
Enterobacter aerogenes(10)TBG-MINO   111
DMG-MINO   111
DMG-DMDOT   111
Minocycline   222
Tetracycline   222
Ciprofloxacin≤0.004–0.030.0150.03
Imipenem0.25–20.252
Ceftazidime0.12–>320.12>32
Providencia spp. (10)TBG-MINO4–848
DMG-MINO2–888
DMG-DMDOT4–848
Minocycline4–>3216>32
Tetracycline4–>32>32>32
Ciprofloxacin≤0.004–0.250.030.25
Imipenem0.25–212
Ceftazidime0.03–40.064
Proteus mirabilis (15)TBG-MINO2–848
DMG-MINO1–1648
DMG-DMDOT0.12–211
Minocycline2–32816
Tetracycline1–321632
Ciprofloxacin0.008–0.060.060.06
Imipenem0.003–0.120.060.12
Ceftazidime0.015–0.060.030.03
Proteus vulgaris(15)TBG-MINO1–444
DMG-MINO0.5–412
DMG-DMDOT0.25–10.51
Minocycline0.5–824
Tetracycline0.5–>32832
Ciprofloxacin0.008–0.250.0150.12
Imipenem0.03–0.120.060.12
Ceftazidime0.015–0.250.030.06
Morganella morganii(10)TBG-MINO2–844
DMG-MINO1–444
DMG-DMDOT1–422
Minocycline2–>32416
Tetracycline2–>322>32
Ciprofloxacin≤0.004–10.0080.03
Imipenem2–424
Ceftazidime0.06–320.1232
Pseudomonas aeruginosa (10)TBG-MINO8–161616
DMG-MINO4–888
DMG-DMDOT4–1688
Minocycline2–888
Tetracycline8–>3216>32
Ciprofloxacin0.12–20.252
Imipenem0.5–211
Ceftazidime0.5–32216
Burkholderia cepacia(10)TBG-MINO0.5–424
DMG-MINO0.5–414
DMG-DMDOT0.5–424
Minocycline0.06–20.52
Tetracycline1–>3224
Ciprofloxacin0.03–40.122
Imipenem0.06–848
Ceftazidime0.5–424
Stenotrophomonas malto-philia(10)TBG-MINO1–424
DMG-MINO0.5–412
DMG-DMDOT2–848
Minocycline0.06–0.50.120.25
Tetracycline8–161616
Ciprofloxacin1–424
Imipenem>32>32>32
Ceftazidime4–>328>32
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