Table 2.

Summary of purification of metal-dependent β-lactamase of P. aeruginosa 101/1477 produced inE. coli BL21(DE3)a

Product or purification stepVol (ml)Amt of protein (mg)Total activity (μmol min−1)Yield (%)
Crude extract15038041,800100
S-Sepharose FF column elution804940,80097
MonoS column elution203539,00093
  • a Standard deviations were ±10%.