Table 1.

Experimental conditions for kinetic studies

AntibioticConcn range (μM)Δɛ (M−1cm−1)λ (nm)Enzyme (μg)
Benzylpenicillin500–1,000−7752350.5–1
Ampicillin250–1,000−8202351–2
Carbenicillin800–1,500−7802351–2
Piperacillin500–2,000−8202351–10
Temocillin100–1,200−6602355–10
Ticarcillin100–1,200−660235<10
Imipenem50–120−9,0003000.02–0.05
Meropenem100–200−6,5003000.5–2
Panipenem50–100−7,4003000.02–0.5
Biapenem100–200−7,6002931–2
Nitrocefin50–150+15,0004820.05–0.5
Cephaloridine25–150−10,0002600.5–1
Cephalothin25–150−6,5002600.5–1
Cefuroxime50–150−7,6002600.5–1
Ceftazidime50–120−9,0002605–10
Cefotaxime50–100−7,5002601–5
Desacetyl-cefotaxime50–100−7,2502600.05–1
Cefoxitin25–100−7,7002600.2–0.5
Cefpirome50–100−4,5002600.5–2
Cefepime25–75−10,0002601–3
Loracarbef50–100−10,6002600.05–0.5
Moxalactam50–100−4,0002600.5–1
Aztreonam1,000−700320 5
Carumonam1,000−810310 5