Table 1.

Classification based on PBP 2X sequence differences and properties of S. pneumoniae strains

GroupStrainSerotypeMIC (μg/ml)aAmino acid motifbPCR resultc
PCCTXCPDXCDTRCFDNCCLS T M KA H S S N VL K S G Tpbp1apbp2b
None1/E9150.0310.0310.1250.0160.1251–––––––––––––––
2/HSB1150.0310.0310.1250.0310.1252––––V––––––––––
3/YO4230.0310.0630.1250.0310.1251–––––––––––––––
4/HSC2160.0310.0630.1250.0310.251–––––––––––––––
5/H48190.0310.0630.1250.0310.251–––––––––––––––
I6/HSB530.0080.1250.50.0310.252––––––––––––––A
7/T3760.0080.1250.250.0310.252––––––––––––––A
8/TJ2490.0160.1250.250.0310.251––––––––––––––A
9/E1390.0160.250.50.0310.252––––––––––––––A
II14/TO22190.0310.0630.250.0630.1250.5–––––L––––V––––
17/SU1190.0630.1250.250.0160.251–––––L––––V––––
21/H3100.0310.1250.250.0630.1251–––––L–––––––––
16/MA37230.0630.1250.250.1250.251––––VL–––––––––
22/H23140.0310.0630.250.0630.251––––VL–––––––––
III11/KM9930.0310.1250.50.0630.250.5–A–––––––––––––
15/HSC930.0310.1250.50.1250.251–A–––––––––––––
18/TJ4130.0310.1250.50.0630.252–A–––––––––––––
23/H6930.0310.250.250.0630.251–A–––––––––––––
25/S1930.0310.1250.50.1250.251–A–––––––––––––
IV34/H31610.510.5432–A––––––––V––––++
36/Z21620.520.5864–A––––––––V––––++
37/H281910.520.5264–A––––––––V––––++
38/KM901920.520.5464–A––––––––V––––++
39/Z131920.510.5464–A––––––––V––––++
40/H292310.520.5464–A––––––––V––––++
41/B992310.520.5464–A––––––––V––––++
42/H0144141864–A––––––––V––––++
27/S46230.50.510.25232–A––––––––V––––+
1A22/HA560.250.510.25216–A––––––––V––––+
V20/SHA319241643264–A––V–––––V––––++
29/KK13323281643264–AF–––––––V––––++
30/NG4423221621664–AF–––––––V––––++
31/KU5231282864–AF–––––––V––––++
32/KU8119441621664–AF–––––––V––––++
33/AK5140.125416228–AF–––––––V––––+
  • a Susceptibilities to PC, CTX, cefpodoxime (CPDX), cefditoren (CDTR), cefdinir (CFDN), and cefaclor (CCL) were determined by previously described methods (1).

  • b Only amino acid residues differing from PBP 2X conserved motif sequences of the PSSP strain R6 are shown. Conserved amino acid motifs are underlined.

  • c Altered PBP genes were detected by PCR as described previously (29). +, altered; −, not altered.