Table 2.

Susceptibilities to β-lactam antibiotics of S. pneumoniae transformants obtained with pbp2x DNA

StrainaMIC (μg/ml)b
PCCTXCPDXCDTRCFDNCCL
Recipient, KK970.0310.0160.0310.0160.0630.5
Transformant
 KK972X34/H310.0630.52.00.54.02.0
 KK972X40/H290.0630.52.00.54.02.0
 KK972X29/KK1330.0631.02.00.58.02.0
 KK972X32/KU810.0631.04.00.58.02.0
  • a Nonencapsulated strain KK97 was transformed with PCR-amplified pbp2x from group IV and V strains as the donor DNA. Transformants were selected on plates containing 0.2 or 0.5 μg of CTX per ml. Example of transformant nomenclature, KK972X34/H31 corresponds to KK97 strain containing the pbp2x gene from strain 34/H31.

  • b For abbreviations of β-lactams, see Table1, footnote a .