TABLE 1

Kinetic parameters for PAS uptake by stably transfected HEK-OATP1B1, HEK-OCT1, HEK-OCT2, HEK-OAT1, and HEK-OAT3 cellsa

TransporterKm (μM)Vmax (pmol/min/mg protein)
OATP1B150.0 ± 13.6129.7 ± 17.5
OCT120.3 ± 4.648.7 ± 12.2
OCT228.7 ± 6.870.7 ± 9.8
OAT178 ± 18.21,898 ± 112
OAT3100.0 ± 23.61,572 ± 207
  • a The intracellular kinetics of PAS uptake were derived from an in vitro experiment with PAS at concentrations ranging from 1 to 400 μM, and the data are normalized to those for the control. Data are presented as the means ± SDs from three or more independent experiments. Km, Michaelis-Menten constant; Vmax, maximum rate of uptake.