Table 2.

PCR primers used to characterize the vanB gene cluster in VRE strains used in this study

PCRProduct size (bp)Primer sequence (5′-3′)Locationa
vanB1 b 433GTG ACA AAC CGG AGG CGA GGA5,434–5,454
CCG CCA TCC TCC TGC AAA AAA5,866–5,846
vanBconsensusc 484CAA AGC TCC GCA GCT TGC ATG5,340–5,360
TGC ATC CAA GCA CCC GAT ATA C5,823–5,802
vanSB-vanYB 309ATA TGC GCT GGA AAA CAC CTC2,114–2,134
CCC CAG ATT GTT TCA TAT GCC2,422–2,402
vanBlongd 5,959GTT TGA TGC AGA GGC AGA CGA CT450–472
ACA AGT TCC CCT GTA TCC AAG TGG6,408–6,385
  • a The nucleotide positions given are according to the published sequence of the V583 vanB gene cluster (8).

  • b These primers are described by Clark et al. for a PCR designated vanB (6) which amplifies thevanB1 gene.

  • c This vanB PCR amplifies all known subtypes of the vanB gene.

  • d These primers are described by Haaheim et al. (13).