Table 1.

Bacterial strains and plasmids

Strain or plasmidRelevant properties and commentsaSource or reference(s)
Strains
E. coli
  DH5αrecA endA1 gyrA96 thi-1 hsdR17 supE44 relA1 φ80 lacZΔM15Bethesda Research Laboratories
  SN01ampA1+ ampC+pyrB thr leu recA rpsL25
  SN03ampA1 ampC8 pyrB thr leu recA rpsL; spontanous SN01 mutant (mutations in structural and regulatory regions of ampC)25
  K-12 XACNar Rir41
  TKH11E. coli XAC transformant from plasmid preparation of KH1142
  TNBL63E. coli XAC transformant from plasmid preparation of NBL6342
K. oxytoca
  KH11Azr Xmr clinical isolate41, 42
  KH55AzrXmr clinical isolate41, 42
  KH78Azr Xmr clinical isolate41, 42
  KH93AzrXmr clinical isolate41, 42
  NBL63Azr Xmr clinical isolate41, 42
Plasmids
 pGEM-3ZAmpr subcloning vectorPromega
 pNU6Ampr harboringampC10
 pKH11Plasmid fromK. oxytoca KH11 (subgroup 2)41, 42
 pNBL63Plasmid from K. oxytoca NBL63 (subgroup 3)41, 42
 pTKH11Plasmid from transformant TKH1142
 pTKH78Plasmid from transformant TKH7842
 pTNBL63Plasmid from transformant TNBL6342
 pSW-21pGEM-3Z with 0.85-kbPstI fragment of pTKH11This study
 pSW-22pGEM-3Z with 2.74-kb PstI-BglII fragment of pTKH11This study
 pSW-24pGEM-3Z with 2.24-kbBglII fragment of pTKH11This study
 pSW-28pGEM-3Z with 5.96-kb BglII fragment of pTKH11This study
 pSW-26pGEM-3Z with 2.45-kbBglII fragment of pNBL63This study
  • a Amp, ampicillin; Az, aztreonam; Na, nalidixic acid; Ri, rifampin; Xm, cefuroxime.