Table 3.

Steady-state kinetic parameters of the purified FAR-1 β-lactamase

Substratekcat(s−1)Km(μM)kcat/Km (μM · s−1)
Benzylpenicillin165305.5
Amoxicillin190503.8
Ticarcillin49.5311.6
Piperacillin412459.2
Cephalothin141040.13
Cephaloridine131>500<0.1
Cefuroxime3.3>500<4 × 10−3
CeftazidimeNHa NDb ND
Cefotaxime5>500<6 × 10−3
Aztreonam13.2400<0.02
  • a NH, not hydrolyzable.

  • b ND, not detectable.