Table 1.

Bacterial strains and plasmids used in this study

Strain or plasmidRelevant genotype or phenotypeSource or reference
E. coliJM109 endA1 hsdR17 gyrA96 Δ(lac proA) recAB1 relA supE44 thiF′ (lacI q lacZΔM15proAB+ traΔ36)Laboratory collection
pBK-CMV phagemidNeomycinr and kanamycinr Stratagene
N. farcinicaVICThe studied β-lactamaseThis study
N. farcinica 94.0250Common susceptibility phenotypea CIPb
N. farcinica 94.0664Common susceptibility phenotype and AMC intermediateCIP
N. farcinica95.0288Common susceptibility phenotype and AMC intermediateCIP
N. farcinica 95.0684Common susceptibility phenotype and AMC intermediateCIP
N. farcinica96.0027Common susceptibility phenotype and AMC intermediateCIP
N. farcinica 96.0087Common susceptibility phenotype and AMC intermediateCIP
N. farcinica96.0624Common susceptibility phenotypeCIP
N. farcinica 96.0691Common susceptibility phenotypeCIP
N. farcinica 96.0994Common susceptibility phenotype and IMP resistantCIP
N. farcinica 96.1087Common susceptibility phenotypeCIP
N. farcinica97.0244Common susceptibility phenotypeCIP
N. farcinica 3318Common susceptibility phenotypeATCCc
R. equi ATCC 6939No β-lactamase producerATCC
  • a A “common susceptibility phenotype” is amoxicillin, ticarcillin, piperacillin, ceftriaxone, and cefotaxime resistant and amoxicillin-clavulanate (AMC) and imipenem (IMP) susceptible.

  • b CIP, Institut Pasteur Collection, Paris, France.

  • c ATCC, American Type Culture Collection, Rockville, Md.