Table 1.

Bacterial strains used in this study

StrainPlasmidCharacteristicspI(s) of β-lactamases
K. pneumoniaeKUSpMVP-8Clinical isolate from sputum, Kiel, Germany5.4, 7.7
E. coli C600Rifampin resistantNone
E. coliMV1190 lac None
E. coli C600pMVP-8Transconjugant of KUS5.4, 7.7
E. coli MV1190pBCChloramphenicol-resistant cloning vectorNone
E. coliMV1190Transformant of KUS containing a 2.3-kb fragment cloned into pBC7.7