Table 3.

Quinolone susceptibilities of and topoisomerase changes in S. aureus clinical isolates

OrganismStrain no.Results of RFLP analysisaProtein changeMIC (μg/ml)
grlAgyrAGyrAGyrBGrlAGrlBTrovafloxacinCiprofloxacinLevofloxacinSparfloxacinPefloxacin
MSSA108179/80NoneNoneNoneNoneGlu422 to Asp0.0310.250.250.1250.5
MSSA103979/80NoneNoneNoneSer80 to PheNone0.125110.1254
MSSA106684NoneNoneNoneGlu84 to LysSer410 to Pro, Glu422 to Asp0.12520.50.254
MSSA103579/80NoneNoneNoneSer80 to PheNone0.125110.258
MSSA124979/80NoneNoneNoneSer80 to PheNone0.12520.50.258
MSSA130584NoneNoneNoneGlu84 to LysNone0.125110.258
MSSA113479/80NoneNoneNoneSer80 to TyrNone0.25110.258
MSSA87284NoneNoneNoneGlu84 to LysGlu422 to Asp0.12510.50.1254
MSSA164279/80NoneNoneNoneSer80 to PheNone0.25210.258
MSSA127579/8083/84Ser84 to LeuNoneSer80 to PheHis478 to Tyr188864
MSSA164079/8083/84Ser84 to LeuNoneSer80 to TyrNone1841664
MSSA163779/8083/84Ser84 to LeuNoneSer80 to PheHis478 to Tyr4321632128
MSSA162879/8083/84Ser84 to LeuNoneSer80 to PheGlu422 to Asp4256168256
MRSA90679/8083/84Ser84 to LeuNoneSer80 to TyrGlu422 to Asp18816128
MRSA97079/8083/84Ser84 to LeuNoneSer80 to PheAsp432 to Val1323216128
MRSA95479/8083/84Ser84 to LeuNoneSer80 to PheNone264816128
MRSA132679/8083/84Ser84 to LeuNoneSer80 to TyrGlu422 to Asp232816128
MRSA161779/8083/84Ser84 to LeuNoneSer80 to PheNone232816128
MRSA162379/8083/84Ser84 to LeuNoneSer80 to PheNone116416128
MRSA162679/8083/84Ser84 to LeuNoneSer80 to PheNone232816256
MRSA100079/8083/84Ser84 to LeuNoneSer80 to PheGlu422 to Asp132816128
MRSA102079/8083/84Ser84 to LeuNoneSer80 to PheGlu422 to Asp21281616128
MRSA162979/8083/84Ser84 to LeuNoneSer80 to TyrNone18816128
MRSA163079/8083/84Ser84 to LeuNoneSer80 to TyrNone1164864
MRSA162779/8083/84Ser84 to LeuNoneSer80 to PheNone232816256
MRSA115179/8083/84Ser84 to LeuNoneSer80 to PheNone13288256
MRSA125479/8083/84Ser84 to LeuNoneSer80 to PheGlu422 to Asp82563232256
MRSA164379/8083/84Ser84 to LeuNoneSer80 to TyrGlu422 to Asp8643232128
MRSA98379/8083/84Ser84 to LeuNoneSer80 to Tyr, Glu84 to LysGlu422 to Asp161286432256
  • a Codon(s) in which a mutation(s) resulting in loss or acquisition of recognition sites was found.