Peptidoglycan precursors synthesized by E. faecalis BM4405a
| Culture | % Synthesis | ||
|---|---|---|---|
| UDP-MurNAc-tetrapeptide | UDP-MurNAc-pentapeptide[Ala] | UDP-MurNAc-pentapeptide[Ser] | |
| Uninduced | <1 | 100 | <1 |
| Induced (Vmb, 4 μg/ml) | 10 | <1 | 90 |
↵a Cultures were incubated with ramoplanin to inhibit peptidoglycan synthesis for 20 min. Cell extracts were prepared, and precursors were analyzed by high-performance liquid chromatography (4). The individual precursors were characterized by amino acid analysis and mass spectroscopy (19).
↵b Vm, vancomycin.