Table 2.

Peptidoglycan precursors synthesized by E. faecalis BM4405a

Culture% Synthesis
UDP-MurNAc-tetrapeptideUDP-MurNAc-pentapeptide[Ala]UDP-MurNAc-pentapeptide[Ser]
Uninduced<1100<1
Induced (Vmb, 4 μg/ml)10<190
  • a Cultures were incubated with ramoplanin to inhibit peptidoglycan synthesis for 20 min. Cell extracts were prepared, and precursors were analyzed by high-performance liquid chromatography (4). The individual precursors were characterized by amino acid analysis and mass spectroscopy (19).

  • b Vm, vancomycin.