Table 3.

DG-DGGE analysis of CSF samples, patient data, the results obtained by two PCR protocols, PCR–DG-DGGE patterns, and susceptibilities of clinical isolates to RMP

Infection status and patient no.Age (yr)SexaNo. of CSF samplesCSF PCR–DG-DGGE patternbRMP phenotype
TotalPositive by IS6110PCRPositive by rpoB PCR (evaluable by DG-DGGE)
Confirmed CNS TBf by CSF-positive culture
 132Md2 2 1 Wild typeSusceptible
 272M2 2 2 Wild typeSusceptible
 342Fd5 4 4 Wild typeSusceptible
 431F2 1 1 Wild typeSusceptible
 563M4 3 1 Wild typeSusceptible
 630M1 1 1 Wild typeSusceptible
 730Md1 1 1 Wild typeNot available
 835Md1 1 1 Abnormal (A)Resistant
 930Md1 1 1 Abnormal (B)Resistant
 1059Md1 1 1 Abnormal (A)Resistant
 1156Md1 1 1 Abnormal (A)Resistant
 1227Fd2 2 0 Susceptible
 1340F1 1 0 Susceptible
 1434Md1 1 0 Resistant
 1530Md2 2 0 Resistant
 1683Md1 1 0 Not available
 1780F2 2 0 Not available
Confirmed CNS TB at autopsy
 1830Md1 1 1 Abnormal (B)Resistante
 1933Md3 3 2 Abnormal (B)Resistante
 2036Md2 2 1 Abnormal (B)Resistante
Highly probablec
 2127Fd2 2 2 Wild typeNot available
 2230Fd2 2 1 Abnormal (C)Resistante
 2351F2 1 1 Abnormal (C)Not available
 2475M1 1 1 Wild typeNot available
 2530Md1 1 1 Wild typeNot available
 2629M1 1 1 Wild typeNot available
 2729Md1 0 0 Not available
 2841F2 0 0 Not available
Total no. of samples (no. of patients)48 (28)41 (26)26 (20)
% of positive samples (% of patients)85.4 (92.9)54.2 (71.4)
  • a M, male; F, female.

  • b Three different abnormal patterns were found and classified as A, B, or C.

  • c CNS TB was considered highly probable because the suggestive clinical picture was accompanied by at least three of the following supporting tests: (i) compatibly abnormal CSF findings; (ii) computed tomography findings suggesting CNS TB; (iii) evidence of tuberculosis outside the CNS; (iv) clinical response to antituberculosis therapy.

  • d Patients coinfected with human immunodeficiency virus.

  • e Susceptibility testing determined with isolates cultured from respiratory specimens.

  • f TB, tuberculosis.