Table 1.

Bacterial strains and plasmids

Strain or plasmidRelevant characteristic(s)aSource or reference
E. coliJM83araΔ(lacmutant proAB) rpsL[Φ80Δ(lacZ)M15]48
S. maltophilia
 BM2690Ak Gm Is Km Nt TmClinical isolate
 ATCC 13637Ak Gm Is Km Nt TmType strain
Plasmids
 pUC18TraMob Ap48
 pAT680Tra Mob Ak Is Km Nt Tm1.1-kb PstI fragment from BM2690 into pUC18
 pAT681Tra Mob Ak Is Km Nt Tm903-bp EcoRI-PstI fragment from pAT680 into pUC18
  • a Tra, non-self-transferable; Mob, non mobilizable; Ak, amikacin resistance; Ap, ampicillin resistance; Is, isepamicin resistance; Gm, gentamicin resistance; Km, kanamycin resistance; Nt, netilmicin resistance; Tm, tobramycin resistance.