Table 2.

Direct detection of M. tuberculosis rpoB in 84 clinical specimens collected from 62 patients suffering from tuberculosis

Specimen source (no. of specimens)aResultb
SmearCultureIS6110 PCRcrpoB PCR
Respiratory (39)++++
Respiratory (5)+++
Respiratory (1)++
Respiratory (15)+++
Respiratory (7)++
Respiratory (5)+
Nonrespiratory GW and L (6)++++
Nonrespiratory GW, PE, and U (3)+++
Nonrespiratory GW and L (2)+++
Nonrespiratory PE (1)+
  • a Respiratory specimens include sputum and bronchial wash or lavage specimens. Abbreviations: GW, gastric wash; L, lymph node; PE, pleural effusion; U, urine.

  • b +, positive; −, negative.

  • c A previously described homemade nested PCR protocol was used to obtain these results (16).