Table 1.

Oligonucleotide primers used in this study

GeneForward primerReverse primerExpected product size (bp)aPCR program (followed by a 10-min extension step at 72°C)Restriction endonucleases
katA5′-AACCTTTCTTTTTCATCAGCTGGC-3′5′-TTGACAGAGAAAGAATCCCTGA-3′1,19794°C for 30 s, 64°C for 1 min, 72°C for 30 sRsaI, Tsp509I
fdx5′-GATTTAGGGGTTTTAGGCGTGCTAGA-3′5′-AAACTGGCTCGTCTTTTTAAAGACAG-3′47094°C for 30 s, 54°C for 30 s, 72°C for 45 sHpaII, XmaIII
fidA5′-CGATTGGGTGAAAACGCTGTAT-3′5′-TTCTAGCGGGGTTTGGTTGTCT-3′79794°C for 30 s, 55°C for 30 s, 72°C for 30 sDraI, MboI
porγδαβ5′-CACAAGGCGCAAAAAGCCCCTT-3′5′-CTTGTAGGTATGCCCCCATTTTCC-3′3,32994°C for 30 s, 50°C for 30 s, 72°C for 3 minHaeIII, HindIII
rdxA5′-AATTTGAGCATGGGGCAGA-3′5′-GAAACGCTTGAAAACACCCCT-3′85194°C for 30 s, 55°C for 30 s, 72°C for 30 sHhaI, BalI
recA5′-AGTCAAGCGGGAAGACCACT-3′5′-TGCTGATCGCCCATATCCCC-3′28594°C for 1 min, 52°C for 1 min, 72°C for 1 minBfaI, AvaI
sodB5′-CACCATGGGAAACACCATCAAACTT-3′5′-CAATGTAATAAGCATGCTCCCACACATC-3′41794°C for 30 s, 57°C for 30 s, 72°C for 30 sMseI, Sau3AI
  • a Based on the sequenced genome ofH. pylori 26695.