Table 4.

Yield of cells and ergosterol content of a 100-ml culture under stationary growth conditions

Method (units of measurement)Avg yield ± SDa (itraconazole IC50 [μM])
C43C48C56
Coulter counter (108cells)201 ± 24 (0.03)127 ± 10b (0.07)111 ± 6b (0.1)
UV analysis (μg of Δ5,7-diene sterol)1,068 ± 125 (0.02)1,049 ± 169 (0.1)921 ± 81 (0.12)
Sterol/cell ratio (fg/cell)53 ± 882 ± 983 ± 9
  • a Based on at least six measurements.

  • b P < 0.05 as determined by Wilcoxon statistical analysis.