Table 1.

Bacterial strains and plasmids used in this studya

Strain or plasmidDescriptionSource or reference
Strains
P. aeruginosa
  PAO1Wild typeP. Phibbs, S. Lory, University of Washington
  PAO200PAO1 with unmarked Δ(mexAB-oprM)H. Schweitzer (41)
  2547Spontaneous tobramycin-resistant derivative of PAO1This study
  2548Spontaneous tobramycin-resistant derivative of 2547This study
  3579PAO1 with unmarked Δ(amrR)This study
  35802547 with unmarked Δ(amrB)This study
  3582PAO1(pAMR-1)This study
  3583PAO1(pUCP20) plasmid control strainThis study
  37373579(pXZL34)This study
  913CF clinical isolateCHMC
  1030CF clinical isolateCHMC
  1085CF clinical isolateCHMC
  1104CF clinical isolateCHMC
  1151CF clinical isolateCHMC
  1168CF clinical isolateCHMC
  1200CF clinical isolateCHMC
  1249CF clinical isolateCHMC
  1250CF clinical isolateCHMC
  1365CF clinical isolateCHMC
  1452CF clinical isolateCHMC
  1457CF clinical isolateCHMC
  1520CF clinical isolateCHMC
E. coli
  DH5αF f80dlacZΔM15 Δ(lacZYA-argF)U169 deoR recA1 endA1 hsdR17(rK , mK +) phoA supE44 λ thi-1 gyrA96 relA1 Gibco-BRL
  DH10BF mcrAΔ(mrr-hsdRMS-mcrBC) ψ 80dlacZΔ M15 ΔlacX74 deoR recA1 endA1 araD139 Δ(ara,leu)7697 galU λ rpsL nupG Gibco-BRL
  S17.1λpir thi pro hsdR recA Tra+ P. Phibbs, S. Lory, University of Washington
  SM10Kmr; mobilizer strain (thi-1 thr leu tonA lacY supE recA::RP4-2Tc::Mu)H. Schweizer, Colorado State University (42)
Plasmids
 pGEM-TApr; T-tailed cloning vectorPromega Corp.
 pUCP20Apr Cbr,ori1600 +; broad-host-range cloning vector (GenBank no. U07165)H. Schweizer (47)
 pAMR-1pUCP20 derivative carrying the amrAB genes on a 4.9-kb EcoRV-EcoRI fragmentThis study
 pXZL34pVLT31 derivative carrying the oprM gene under the control of an IPTG-inducible promoterR. Hancock, University of British Columbia (48)
 pEX18TApr Cbr;sacB+ oriT +; gene replacement vector (GenBank no. AF004910)H. Schweizer, Colorado State University (42)
 pPS858Apr Gmr; source of Gmr-GFP fragment flanked by FRT sitesH. Schweizer (17)
 pFLP2Apr Cbr; source of yeast Flp recombinase (GenBank no. AF048702)H. Schweizer (17)
 pEX-AMRRpEX18T derivative used for the deletion of the amrR geneThis study
 pEX-AMR3pEX18T derivative used for the deletion of theamrAB genesThis study
  • a Apr, ampicillin resistant; Cbr, carbenicillin resistant; Gmr, gentamicin resistant; Kmr, kanamycin resistant; CHMC, Children’s Hospital and Medical Center, Seattle, Washington; GFP, green fluorescent protein structural gene; FRT, yeast Flp site-specific recombinase recognition sequence.