Table 1.

Bacterial strains

StrainRelevant genotypeaSource or reference
DM4100Wild type 20
KD66DM4100 gyrA (NalrS83L) 17
KD1366 gyrA(Nalr S83L) parC (CiprS80L)b 25
KD1373DM4100 parC (CiprS80L) 25
KD1525 gyrA(Nalr D82A) parC (Cipr S80L)This work, as described in reference 25
KD1721DM4100 gyrA(Nalr A51V)This work, by P1-mediated transduction from T/r (6)
KD1911DM4100 gyrA(Nalr A67S)This work, by P1-mediated transduction from P-10 (22)
KD1915DM4100 gyrA(Nalr G81C)This work, by P1-mediated transduction from N-97 (22)
KD1909DM4100 gyrA(Nalr S83W)This work, by P1-mediated transduction from P-18 (22)
KD1913DM4100 gyrA(Nalr D87N)This work, by P1-mediated transduction from N-113 (22)
KD1917DM4100 gyrA(Nalr Q106H)This work, by P1-mediated transduction from N-89 (22)
KD1957 gyrA (NalrA51V) parC (Cipr S80L)This work, by P1-mediated transduction from KD1721zeg::Tn10c into KD1373
KD1959 gyrA (Nalr A67S) parC(Cipr S80L)This work, by P1-mediated transduction from KD1911 zeg::Tn10 into KD1373
KD1961 gyrA (Nalr G81C) parC(Cipr S80L)This work, by P1-mediated transduction from KD1915 zeg::Tn10 into KD1373
KD1963 gyrA (Nalr S83W) parC(Cipr S80L)This work, by P1-mediated transduction from KD1909 zeg::Tn10 into KD1373
KD1965 gyrA (Nalr A84P) parC(Cipr S80L)This work, by P1-mediated transduction from KD1975 zeg::Tn10 into KD1373
KD1967 gyrA (Nalr D87Y) parC(Cipr S80L)This work, by P1-mediated transduction from KD1977 zeg::Tn10 into KD1373
KD1969 gyrA (Nalr Q106H) parC(Cipr S80L)This work, by P1-mediated transduction from KD1917 zeg::Tn10 into KD1373
KD1971 gyrA (Nalr D87N) parC(Cipr S80L)This work, by P1-mediated transduction from KD1913 zeg::Tn10 into KD1373
KD1973DM4100 gyrA (Nalr D82A)This work, by P1-mediated transduction from KD1525 into DM4100 (25)
KD1975DM4100 gyrA (NalrA84P)This work, by P1-mediated transduction from P-5 (22)
KD1977DM4100 gyrA (NalrD87Y)This work, by P1-mediated transduction from KD1677d (M. Friedman)
  • a Amino acid abbreviations: A, alanine; D, aspartic acid; C, cysteine; H, histidine; G, glycine; L, leucine; N, asparagine; P, proline; Q, glutamine; S, serine; V, valine; W, tryptophan; Y, tyrosine. The first letter represents the wild-type amino acid at the position indicated by the number. The second letter indicates the identity of the amino acid which was substituted for the original at that position.

  • b By itself this parC allele confers no resistance because topoisomerase IV is a secondary target.

  • c zeg::Tn10 encodes a tetracycline resistance gene that is 50% cotransducible withgyrA.

  • d Obtained by selection on agar containing nalidixic acid (20 μg/ml).