Table 1.

Epidemiology, phenotypes, and β-lactamase genes ofK. pneumoniae isolates collected in 1994 and 1996

Isolate (yr collected)Date collected (day/mo)WardSourceEtest MIC ratioaPFGE typePlasmid size (kb)β-Lactamase(s)
Gene hybridizationbpI value(s)Sequence(s) found
79 (1994)04/10ICUCentral venous catheter16A1401 × TEM5.6TEM-63
871 × SHV6.3
7.6SHV-19
113 (1994)No dataNo dataBlood>32A1362 × TEMTEM-53
1031 × SHV5.6
807.6SHV-19
63
114 (1994)No dataNo dataBlood>32A1362 × TEMTEM-53
1031 × SHV5.6
807.6SHV-19
63
122c (1994)09/10ICUCentral venous catheter<2A1421 × TEM5.4TEM-1
1111 × SHV7.6SHV-19
88
72
7
202c (1994)12/10ICUPeritoneal fluid>32A11861 × TEM5.6TEM-63
1111 × SHV7.6SHV-19
10 (1994)27/09SurgicalPus>32A11402 × TEM5.6TEM-63
411 × SHV6.3
7.6SHV-19
73 (1994)02/10OrthopedicPus8A21401 × TEM5.6TEM-53
411 × SHV6.3
7.6SHV-19
91 (1994)No dataNo dataBlood culture>32A31361 × TEM5.6TEM-63
1031 × SHV7.6SHV-19
35
117c (1994)05/10ICUPus (abdomen)<2A31031 × TEMTEM-53
801 × SHV7.6SHV-19
63
35
30
118c (1994)05/10ICUPus (abdomen)<2C1361 × TEMTEM-53
1031 × SHV7.6SHV-19
80
63
97 (1994)No dataNo dataBlood<2B1362 × TEMTEM-1
1031 × SHV7.6SHV-19
571 (1994)No dataNo dataBlood8D1401 × TEM5.4TEM-1
872 × SHV5.6
716.3
596.8
497.6SHV-2 and -21
41
35
4291e (1996)28/06ICUEndotracheal aspirate64A1393 × TEMTEM-53
882 × SHV5.4
505.6
6.3
6.8
7.6SHV-1 and -2
4495 (1996)08/07ICUEndotracheal aspirate>128A41393 × TEM5.4TEM-1 (2)f
1102 × SHV5.6
597.6SHV-1
8.2SHV-5
4265d (1996)27/06ICUEndotracheal aspirate>128E1422 × TEM5.4TEM-1 (2)
2 × SHV5.6
6.3
7.6SHV-1
8.2SHV-5
4744d (1996)18/07NurserySputum>128E1422 × TEM5.4TEM-1 (2)
2 × SHV6.3
7.6
8.2SHV-5 (2)
8143d (1996)29/06ICUBlood>128E1422 × TEM5.4TEM-1 (2)
2 × SHV5.6
7.6
8.2SHV-5 and -22
4120 (1996)20/06ICUEndotracheal aspirate32F872 × TEM5.4TEM-1 (2)
712 × SHV6.3
596.8
7.6SHV-2 (2)
4448 (1996)05/07NurseryPus (drip site)64F11393 × TEMTEM-53
1102 × SHV5.4
595.6
507.6SHV-19
8.2SHV-5
4175e (1996)22/06ICUEndotracheal aspirate4G1401 × TEM5.4TEM-1
872 × SHV6.3
76.8
57.6SHV-1 (2)
4183d (1996)22/06ICUEndotracheal aspirate>128H1391 × TEM5.4TEM-1
1102 × SHV5.6
506.3
7.6SHV-20
8.2SHV-5
4634 (1996)No dataNo dataNo data>64I1393 × TEM5.4TEM-1
1102 × SHV6.8
507.6SHV-2 (2)
238.2
4669 (1996)17/07NurseryPleural cavity tap>32J1393 × TEM5.4TEM-1 (3)
1102 × SHV6.3
727.6SHV-2 and -20
598.2
4700 (1996)17/07PediatricUrine>64K1862 × TEM5.4
1112 × SHV5.6TEM-63 (2)
6.3
7.6SHV-1 (2)
4824 (1996)22/07ICUEndotracheal aspirate8L1422 × TEM5.4TEM-1 (2)
592 × SHV7.6SHV-1 and -20
7
  • a Ratio of MIC of ceftazidime to the MIC of ceftazidime plus clavulanate at 4 μg/ml, as determined by the Etest.

  • b Numbers of SHV genes were estimated from Southern blots of whole genomic DNA digested with SalI and hybridized with a SHV-specific probe, and the numbers of TEM genes were estimated from Southern blots of whole genomic DNA digested withBamHI, HindIII, or HincII and hybridized with a TEM-specific probe.

  • c Four isolates from patient I.

  • d Four isolates from patient II.

  • e Two isolates from patient III.

  • f Numbers in parentheses indicate the number of copies of the gene encoding the β-lactamase indicated.