Table 3.

Nucleotide and amino acid sequence changes of TEM-63, SHV-19, SHV-20, SHV-21, and SHV-22a

EnzymeNucleotide positionsbNucleotide changeAmino acid positioncAmino acid change
TEM-63555–57CTT→TTT21Leu→Phe
304–306GAG→AAG104Glu→Lys
484–486CGT→AGT164Arg→Ser
538–540ATG→ACG182Met→Thr
SHV-19505–507CTT→TTT173Leu→Phe
SHV-20505–507CTT→TTT173Leu→Phe
700–702GGC→AGC238Gly→Ser
SHV-21352–354CTC→TTC122Leu→Phe
505–507CTT→TTT173Leu→Phe
700–702GGC→AGC238Gly→Ser
SHV-22460–462AAC→AAG158Asn→Lys
700–702GGC→AGC238Gly→Ser
703–705GAG→AAG240Glu→Lys
  • a Abbreviations: A, adenine; C, cytosine; G, guanine; T, thymine; Ala, alanine; Asn, asparagine; Glu, glutamate; Leu, leucine; Lys, lysine; Phe, phenylalanine; Ser, serine; Gly, glycine.

  • b Nucleotide numbering is in accordance with the AGT codon start of previously reported SHV sequences.

  • c Amino acid numbering is according to Ambler et al. (1).