Table 1.

Buffers used in ion exchange chromatography

Isolate and source Ion-exchange column and buffer
04737, BelgiumCM-Sephadexain 10 mM sodium phosphate, pH 6.6
A-15, KuwaitA-Sephadex in 20 mM Tris HCl, pH 8.2
A-Sephadex in 20 mM Tris HCl, pH 7.8b
I-16, SingaporeCM-Sephadex in 50 mM malonic acid-malonate, pH 5.6
327009, SpainCM-Sephadex in 10 mM sodium phosphate buffer, pH 6.8
  • a Sephadex was obtained from Pharmacia.

  • b Second purification step, performed after dialysis of the enzyme against 20 mM Tris HCl buffer, pH 7.8.