Table 2.

Kinetic parameters of CTX-M-10

AntibioticRelative hydrolysis ratea(%)Relative Vmax(%)Km (μM)RelativeVmax/Km ratioIC50 (μM)
Penicillin G10010034100
Cephaloridine1672009671
Cefuroxime951003888
Cefotaxime81502861
Ceftazidime NHb NDc NDND
Cefepime5NDNDND
Clavulanic acid0.006
Tazobactam0.0009
Sulbactam0.32
  • a Hydrolysis rates (micromoles per minute per microliter) were determined using a 100 μM concentration of each substrate; relative values were determined by considering the penicillin G rate to be 100.

  • b NH, no hydrolysis detected.

  • c ND, not done.