Table 1.

Most important strains and plasmids used in this study

Strain or plasmidDescriptionReference or source
Strains
S. viridochromogenes Tü57Wild-type strain, avilamycin producer7, 14
S. lividans
  TK66Strain devoid of plasmid16
  TK66 (pUWL201) (GWO)TK66 harboring plasmid pUWL201This study
  TK66 (pUWL201-Ra) (GWRa)TK66 harboring plasmid pUWL201-RaThis study
  TK66 (pUWL201-Rb) (GWRb)TK66 harboring plasmid pUWL201-RbThis study
  TK66 (pUWL201-ABC) (GWABC)TK66 harboring plasmid pUWL201-ABCThis study
  TK66 (pUWL201-RbRa) (GWRbRa)TK66 harboring plasmid pUWL201-RbRaThis study
  TK66 (pUWL201-RbRaABC) (GWRbRaABC)TK66 harboring plasmid pUWL201-RbRaABCThis study
E. coli
  XLIBlueHost strain for cloning experimentsStratagene
  BL21 (DE3)pLysSHost strain for expression experimentsInvitrogen
Plasmids
 pWHM3 E. coli-Streptomyces shuttle vector, used for expression experiments in Streptomyces 27
 pSG116-kb PstI fragment cloned into the vector pWHM3This study
 pUWL201 E. coli-Streptomyces shuttle vector, used for expression experiments in Streptomyces 26
 pUWL201-RaPlasmid containing the cloned aviRageneThis study
 pUWL201-RbPlasmid containing the clonedaviRb geneThis study
 pUWL201-ABCPlasmid containing the cloned aviABC1/2 genesThis study
 pUWL201-RbRaPlasmid containing aviRa andaviRb This study
 pUWL201-RbRaABCPlasmid containing aviRa, aviRb, and aviABC1/2 This study
 pRSETbVector used for expression experiments inE. coli Invitrogen
 pRSETb-RaPlasmid containing the cloned aviRa geneThis study
 pRSETb-RbPlasmid containing the cloned aviRbgeneThis study