Table 3.

Spectra of spontaneous mutations

Mutant strainNo. of independent mutations analyzedMutant allelesaMIC (μg/ml)
TotalDistributionDesignationNucleotide changeAmino acid change
UA802 Rifr 606 rpoB1 A to GQ527R256
8 rpoB2 G to AD530N128
7 rpoB3 A to GD530G256
8 rpoB4 A to TD530V128
5 rpoB5 C to TH540Y256
1 rpoB6 C to AL525I256
3 rpoB7 C to AQ527K128
3 rpoB8 C to AH540N64
2 rpoB9 A to TI586P64
2 rpoB10 C to TS526L32
3 rpoB11 A to GH540R128
2 rpoB12 G to TD530Y32
4 rpoB13 C to TL525P128
3 rpoB14 T to AI586N128
3?UnknownUnknown64
26695 Rifr 41 rpoB2 G to AD530N128
2 rpoB3 A to GD530G256
1 rpoB4 A to TD530V128
UA802 Clar 42 23S1 A2142GNA16
2 23S2 A2143GNA4
UA802 Cipr 121 gyrA1 A to CD91A4
4 gyrA2 G to TD91Y8
5 gyrA3 G to AD91N8
2 gyrA4 A to GD91G8
11639 Mtzr 41 rdxA1 C to TR16C32
2 rdxA2 +AShift at 64, stop at 7364
1 rdxA3 C to TP51 L64
11639 Mtzr (serial passage)185 rdxA2 +AShift at 64, stop at 7364
2 rdxA4 −TShift at 72, stop at 7664
1 rdxA5 +AGShift at 41, stop at 55128
1 rdxA6 −GShift at 133, stop at 13764
1 rdxA7 G to TE175 stop32
1 rdxA8 −AShift at 35, stop at 5564
1 rdxA9 G to AC87Y64
1 rdxA10 +AShift at 9, stop at 23128
1 rdxA11 C to TA67V16
1 rdxA12 +14 ntShift at 70, stop at 80128
1 rdxA13 T to CY47H64
2?UnknownUnknown64
  • a When a distinct mutation was identified, it was given an allele name (designation), which represents a specific change at the indicated nucleotide (nt) in a gene and a corresponding amino acid change in the gene product.