Table 3.

Substrate profile for KPC-1

Substrate kcat (s−1)Relative kcatKm (μM) Relativekcat/Km
Cephaloridine340 ± 16 100560 ± 97 100
Cephalothin 75 ± 7 2253 ± 0.3 240
Nitrocefin 78 ± 2 2324 ± 5 530
Cefotaxime 14 ± 1 4.2160 ± 19 15
Cefoxitin 0.26 ± 0.01 0.08120 ± 17 0.4
Ceftazidime 0.10 ± 0.02 0.0394 ± 1.8 0.2
Benzylpenicillin 32 ± 1 9.623 ± 2.3 230
Ampicillin 110 ± 4 33130 ± 21 140
Cloxacillin 25 ± 1 7.4100 ± 35 41
Piperacillin 24 ± 0 7.218 ± 3 230
Imipenem 12.4 ± 0 3.781 ± 0.4 26
Meropenem 3.0 ± 0.1 0.912 ± 2 41.5
Aztreonam 20 ± 1 5.9310 ± 35 11