Table 2.

Amino acid differences in DHFR sequences of trimethoprim-resistant and -susceptible isolates

IsolateAmino acid at positiona:MIC (mg/liter)
14*1620*265360*70*74*7881*838691*92*94*100*111120133135*147149*164
ATCC 49619EVEHMKPIAQVVQDEIPHELFAK4
CP1015L4
Pn93/1791IK4
Pn93/917DAA8
Pn93/908DAA4
Pn94/258AA4
Pn93/720DDSLHHADLFSTE256
Pn93/1293DYSTYSDLFSS256
Pn93/1802DYIQSTALQFST256
P48DSHIADLQFST256
R12DYIQSTALQFST512
  • a *, positions at which amino acids are altered in all trimethoprim-resistant isolates reported by Hartman et al. (37th ICAAC), Adrian and Klugman (1), Pikis et al. (15), and the present study; —, amino acid is the same as in ATCC 49619.