Table 2.

Phenotypic properties of different S. aureusstrains

StrainRelevant genotypeaMIC (μg/ml)bςBactivitycColor
TeicoplaninVancomycin
MHBHIMHBHI
MB33BB255;asp23p::pECasp23p-luc+262434.0 ± 3.00White
MB49MB33;rsbU + V + W + sigB + 48–123 4–6301.3 ± 23.8Orange
MB118MB33;rsbW1 8243 6632.5 ± 13.8Orange
MB119MB33;rsbW2(Am)12384–68609.0 ± 43.5Orange
MB127MB33;rsbW3 12324–68760.3 ± 52.8Orange
MB128MB33;rsbW4 6243 6933.8 ± 99.5Orange
MB130MB33;rsbW5(Am)8244 8869.5 ± 74.5Orange
MB132MB33;rsbW6 8324 8804.0 ± 70.5Orange
MB137MB33;sigB2 12324 8671.0 ± 35.5Orange
MB138MB33;rsbW7 12323–481,084.0 ± 46.5Orange
MB140MB33;chrX1 8163 631.2 ± 2.80White
MB148MB33;rsbW8 12324 8883.8 ± 70.3Orange
MB158MB137;sigB1(Am)242 40.59 ± 0.08White
MB159MB137;crtM::pBTcrtM 12324 8704.8 ± 43.8White
MB161MB140;rsbU + V + W + sigB + 183238315.3 ± 24.3Orange
MB162MB140;sigB1(Am)816360.66 ± 0.07White
MB163MB140;crtM::pBTcrtM 8163641.6 ± 3.80White
MB213MB119;rsbU + V + W + sigB + 48–1234–6313.0 ± 12.8Orange
MB215MB119;sigB1(Am)24240.57 ± 0.09White
Newman rsbU + V + W + sigB + 2624354.5 ± 31.5Yellow
MSSA1112 rsbU + V + W + sigB + 1.541.56272.4 ± 27.3Yellow
  • a Detailed relevant genotypes and phenotypes are listed in Table 1; exact mutation sites are listed in Table 3. chrX1, unmapped mutation.

  • b MICs were obtained using Etest as outlined in Materials and Methods. MH, Mueller-Hinton agar.

  • c ςB transcriptional activity (relative light units) was determined from cells grown to an OD600 of 1.5 by measuring the luciferase activity of Luc+, the product of the luc+ reporter gene fused to the ςB-dependent promoters of asp23 (asp23p). The values shown are the results of four independent assays.