Table 1.

Growth phenotypes and MICs of mutants obtained via serial passage or chemical mutagenesisa

StrainClassGrowth phenotype on:MIC (μg/ml)
Liquid media (OD600)Solid media (colony size)
MHBCDMMHASCVDaptoVancAmpNisinGent
Sa42WT6.056.6++++++0.780.786.253.1250.78
Sa278b 15.937.87++++++12.51.566.25250.78
Sa28414.976.38++++++6.251.566.25250.78
Sa28521.23NG++++6.250.783.13250.39
Sa28630.63NG++6.250.39ND6.250.78
Sa28713.896.59++++++6.251.566.25251.56
Sa28815.348.17++++++6.250.78ND250.78
Sa28930.690.45++12.50.39ND3.125ND
Sa29030.91NG++12.50.39NDND1.56
Sa29130.71NG++250.391.563.1250.78
Sa29231.37NG++12.50.78NDND0.78
Sa29330.43NG++12.50.78NDND1.56
Sa29521.28NG++++12.51.566.256.25<0.1
  • a Abbreviations: OD600, optical density at 600 nm; SCV, MHA plus hemin, thiamine, and menadione; Dapto, daptomycin; Vanc, vancomycin; Amp, ampicillin; Gent, gentamicin; WT, wild type; NG, no growth, 16 h; ND, not determined.

  • b Obtained via serial passage techniques.