Table 4.

Structure of mec complex of and methicillin MIC for mutants derived from S. haemolyticus strain SH621

StrainPBP2 productionaSelective methicillin concn (mg/liter)bClass of meccomplexcPCRdstructure of:MIC of methicillin (mg/liter)
mecImecR1
PBMS
SH621 A ++ + 2
SH621-15 + 1 A+ + + 8
SH621-16 + 1 A + ++ 4
SH621-32 + 3 A + + + 512
SH621-34 + 3 A + + + <1,024
SH621-310 + 3 A + + + 512
SH621-81+ 8 A + + + <1,024
SH621-82 +8 A + + + <1,024
SH621-83 + 8A + + + 1,024
SH621-84 + 8 A ++ + 1,024
SH621-85 + 8 A + + +<1,024
SH621-86 + 8 A + + +<1,024
SH621-87 + 8 A + + + 1,024
SH621-88 + 8 A + + + 1,024
SH621-89+ 8 A + + + <1,024
SH621-810 +8 A + + + 512
SH621-17 + 1 C1aΔ + + 16
SH621-37 + 3 C1a Δ+ + 16
SH621-38 + 3 C1a Δ + +16
SH621-39 + 3 C1a Δ + + 16
SH621-13 + 1 C1b Δ + 32
SH621-35 + 3 C1b Δ + 64
SH621-31 + 3 C1b Δ + 32
SH621-33 + 3 C1b Δ + 16
SH621-36 + 3 C1b Δ + 16
SH621-11 + 1 C1c Δ 16
SH621-12 + 1 C1c Δ 16
  • a Judged by the rapid slide latex agglutination assay kit (24).

  • b The concentration of methicillin used for the selection of mutants.

  • c Corresponds to the mec complex shown in Fig. 4.

  • d + indicates that the size of the DNA fragment amplified by PCR was indistinguishable from that of SH621. Δ indicates that the amplified DNA fragment contained a deletion.