Table 1.

Synthetic oligonucleotides primers used for the analysis of mec complex structure

Primer designationSequenceaLocalizationPositionsReference(s)
mA2 5′-AACGTTGTAACCACCCCAAGA-3′ mecA 1461–144111,28
mA4 5′-AGTGTATGATGAGCTATATGAGA-3′ mecA 1959–1981 28
mA55′-CGCTCAGAAATTTGTTGTGC-3′ mecA 212–180 28
mA6 5′-TATACCAAACCCGACAAC-3′ mecA 83–6628
mA7 5′-ATCGTTGACGATAATAGCAATACA-3′ mecA 907–930 28
mA8 5′-ATGTTTGGATTATCTTTATCATAT-3′ mecA 1863–1886 28
iS-15′-ACATTAGATATTTGGTTGCGT-3′ IS431mec 566–5963
iS-2 5′-TGAGGTTATTCAGATATTTCGATGT-3′IS431mec 767–743 3
iS-35′-TCGGATGCTATCATTAAGCAT-3′ IS1272 1668–16882
iS-4 5′-ACAATCTGTATTCTCAGGTCGT-3′ IS1272 1722–1701 2
mI-1 5′-AATGGCGAAAAAGCACAACA-3′ mecI 1923–1942 10, 30
mI-25′-GACTTGATTGTTTCCTCTGTT-3′ mecI 2403–228310, 30
mcR2 5′-CGCTCAGAAATTTGTTGTGC-3′ mecR1 1954–1935 10, 30
mcR3 5′-ATCTCCACGTTAATTCCATT-3′ mecR1 357–376 10, 30
  • a The location of each primer is shown in Fig. 1.