Table 1.

Bacteria and plasmids used in this study

Strain or plasmidRelevant genotype or phenotypeReference or source
P. mirabilis
 BB2000 (JSG785)Spontaneous Rifrmutant; PMr protegrin resistant3
 JSG945BB2000 derivative; PMs protegrin sensitiveThis study
 JSG946BB2000 derivative; PMs protegrin sensitiveThis study
 JSG947BB2000 derivative; PMs protegrin resistantThis study
 JSG948BB2000 derivative; PMs protegrin resistantThis study
E. coli DH5αStratagene
Plasmids
 pBluescript KS+Ampr; cloning vectorStratagene
 pUT/mini-Tn5(Cam)AmprCamr 3
 pWSK29Ampr; cloning vector11
 pWSK30Ampr; cloning vector11
 pWSK945Ampr; 4.5-kb clone of the WT region surrounding the JSG945 Tn5 insertionThis study