Table 2.

Bacterial strains and plasmids used

Strain or plasmidGenotype or phenotypeReference or source
Strains
C. sedlakii2596a Clinical isolate resistant to β-lactamsPresent study
E. coli Top10F mcrA Δ(mrr-hsdRMS-mcrBC)φ80lacZ ΔM15ΔlacX74 deoR recA1 araD139Δ(ara-leu)7697 galU galK rpsL (StrR)endA1 nupG
E. coliK12Rifampin
C. koseriCKBESBLb Clinical isolate
C. koseri CKCESBLClinical isolate
C. koseriCKDESBLClinical isolate
C. koseriCKEESBLClinical isolate
C. koseriCKGESBLClinical isolate
C. koseri CK1Wild typeClinical isolate
C. koseri CK2Wild typeClinical isolate
C. koseri CK3Wild typeClinical isolate
C. koseri CK4Wild typeClinical isolate
C. koseri CK5Wild typeClinical isolate
C. koseri CK7Wild typeClinical isolate
C. koseri CK10Wild typeClinical isolate
C. koseri CIP-82.87Wild type (reference strain)CIP
C. koseriCIP-76.3Wild type (reference strain)CIP
C. koseri CIP-72.8Wild type (reference strain)CIP
C. koseri CIP-72.14PenicillinaseCIP
C. diversus CIP-82.94Wild type (reference strain)CIP
L. malonatica CIP-82.88Wild type (reference strain)CIP
C. sedlakiiCIP-105037Wild type (reference strain)CIP
C. rodentium CIP-104675Wild type (reference strain)CIP
Plasmids
 pBC SK+ ChloramphenicolStratagene
 pBC 2596pBC SK+ + 2.78-kb Sau3AI fragment from genomic DNA of C. sedlakii 2596Present study
  • a The strain was deposited in the CIP under the number CIP-106793.

  • b Phenotype corresponding to penicillin and oxyminocephalosporin resistance by production of ESBLs.