Table 5.

Detection of genes sulI, sulII, andbla by PCR and DNA-DNA hybridizationa

StrainsulIsulIIbla
PCR detectionPlasmid size (kb)Presence of pSCVPCR detectionPlasmid size (kb)Presence of pSCVPCR detectionPlasmid size (kb)Presence of pSCV
OU7085+6No
OU7518+136Yes+136Yes
OU7519
OU7520+140Yes+140Yes
OU7521+90No
OU7522
OU7523
OU7524+136Yes+136Yes
OU7525
OU7526+90No+90No
OU7527+6No
OU7528
OU7529+90No+90No
OU7531+90No+90No
  • a PCR results are the same as those for DNA-DNA hybridization. The results of PCR and DNA-DNA hybridization were consistently in agreement.