Table 4.

MICs of various aminoglycosides for E. colistrain TA531 expressing the indicated 16S rRNAa

AntibioticMIC (μg/ml)
Wild type1406A1408G
Kanamycin A5640<1,280
Neomycin105<1,280
Gentamicin C5320<1,280
Paromomycin105160
G41856405
Amikacin51.25NDb
Apramycin102.5ND 
Tobramycin2.5160ND 
  • a Cells were grown in liquid LB medium containing 200 μg of ampicillin per ml and a twofold dilution (from 1280 to 0.625 μg/ml) of the indicated aminoglycoside. Growth was monitored after 24 h of incubation at 37°C.

  • b ND, not determined.