Table 3.

Susceptibilities to actinonin of S. pneumoniaeR6x strains transformed with wild-type or mutated defB and inhibition of purified His-tagged PDF enzymes by actinonin

S. pneumoniae strainExpressed defBMIC (μg/ml)EnzymeIC50 (nM)
VSPN7035 defB 16Wild type53 ± 6
VSPN7036 defB (Q172K)64Q172K70 ± 8
VSPN7037 defB(A123D)≥64A123D136 ± 16