Table 1.

Oligonucleotide primers used for PCR amplifications

GenePrimeraSequenceProduct size (gene size)Reference
C. freundii ampCfamily ampC15′-ATGATGAAAAAATCGTTATGC-3′1,143 bp14
ampC25′-TTGCAGCTTTTCAAGAATGCGC-3′(Full length)
TEM-1 TEM-1 forward5′-CCCGAATTCGGAAGAGTATGAGTATTC-3′878 bp31
TEM-1 reverse5′-CCCGGATCCCAGTTACCAATGCTTAATC-3′(Full length)
Inserted gene cassettes for type I integrons5′ CS5′-GGCATCCAAGCAGCAAG-3′Variable16
3′ CS5′-AAGCAGACTTGACCTGA-3′
  • a TEM-1 forward encodes anEcoRI restriction site, shown underlined. TEM-1 reverse encodes a BamHI restriction site, shown underlined. CS, conserved sequence.