Table 3.

Fluconazole susceptibilities of S. cerevisiaestrains expressing C. albicans ERG11 genes, as determined by zone of inhibition

Patient no. C. albicans isolateaMean ± SD zone of inhibition (mm)bERG11 allele expressed Amino acid substitution(s)
7 412 (S)43.75 ± 1.12 412K128T
2307 (R)43.08 ± 0.90 2307 K128T
14 1632 (S)42.80 ± 1.93 580 None
1640 (R)44.18 ± 0.75 1640 V437I
15 945 (S)32.50 ± 0.70c 945 D116E, G450E
1619 (R) 0c 1619-1 D116E, G450E, G307S
32.80 ± 1.35c 1619-2D116E, G450E
16 3107 (S)14.50 ± 1.24c 3107 Y132F
3119 (R) 15.57 ± 3.94c 3119Y132F
28 5044 (S)36.81 ± 0.91c 5044 V437I, D446N
5052 (R) 37.56 ± 2.00c 5052V437I, D446N
30 5106 (S) 43.37 ± 1.505106 V437I
5108 (R)30.58 ± 0.79c 5108 V437I, G464S
42 1691 (S) 42.87 ± 0.83 1691 K128T
3731 (R) 0c 3731-1 F126L, K143R
22.33 ± 0.52c 3731-2K143R
51 2274 (S)31.55 ± 1.85c 2274 S405F
2257 (R) 33.16 ± 1.83c 2257S405F
2339 (R) 33.07 ± 1.64c 2339 S405F
59 3917 (S) 42.62 ± 1.503917-1 None
32.62 ± 1.19c 3917-2 F449S
4617 (R)21.05 ± 0.87c 4617 F449S, T229A
4639 (R) 24.37 ± 1.60c 4639F449S, T229A
  • a S, susceptible; R, resistant.

  • b Mean diameters were obtained by measuring the zones of inhibition produced by 10 independent S. cerevisiaeLeu+ transformants. The mean diameter for YKKB-13 transformed with YEp51 alone was 63 mm. The mean diameter for YKKB-13 transformed with a susceptible control was 43 mm. The mean diameter for YKKB-13 transformed with a resistant control was 0 mm.

  • c P < 0.05 .