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diagnostics

  • Detection of <span class="named-content genus-species" id="named-content-1">Candida auris</span> Antifungal Drug Resistance Markers Directly from Clinical Skin Swabs
    Mechanisms of Resistance
    Detection of Candida auris Antifungal Drug Resistance Markers Directly from Clinical Skin Swabs

    Accurate and rapid assessment of Candida auris antifungal drug resistance is crucial for effective infection prevention and control actions, as well as for patient management. Here, performance of a molecular diagnostic platform, enabling rapid identification of FKS1 and ERG11 mutations conferring echinocandin and azole resistance, respectively, was...

    Milena Kordalewska, Annie Lee, Yanan Zhao, David S. Perlin
  • Utility of Targeted, Amplicon-Based Deep Sequencing To Detect Resistance to First-Line Tuberculosis Drugs in Botswana
    Analytical Procedures
    Utility of Targeted, Amplicon-Based Deep Sequencing To Detect Resistance to First-Line Tuberculosis Drugs in Botswana

    Multidrug-resistant tuberculosis (TB) is an alarming threat, and targeted deep sequencing (DS) may be an effective method for rapid identification of drug-resistant profiles, including detection of heteroresistance. We evaluated the sensitivity and specificity of targeted DS versus phenotypic drug susceptibility testing (pDST) among patients starting first-line anti-TB therapy in Botswana. Overall, we found high concordance between DS...

    Qiao Wang, Chawangwa Modongo, Christopher Allender, David M. Engelthaler, Robin M. Warren, Nicola M. Zetola, Sanghyuk S. Shin
  • Utility of Urine Cultures in Predicting Blood Culture Susceptibilities in Patients with Bacteremic Urinary Tract Infection
    Editor's Pick Susceptibility
    Utility of Urine Cultures in Predicting Blood Culture Susceptibilities in Patients with Bacteremic Urinary Tract Infection

    In a review of 428 patients with bacteremic urinary tract infections, urine culture susceptibility results accurately predicted blood culture results when the same organism was isolated from both cultures. Early targeted therapy using urine culture results can potentially reduce broad-spectrum antibiotic exposure and its associated adverse effects and the length of hospitalization.

    Philip W. Lam, J. Bradley Wiggers, Jennifer Lo, Derek R. MacFadden, Nick Daneman
  • Open Access
    Susceptibility
    Validating a 14-Drug Microtiter Plate Containing Bedaquiline and Delamanid for Large-Scale Research Susceptibility Testing of Mycobacterium tuberculosis

    The UKMYC5 plate is a 96-well microtiter plate designed by the CRyPTIC Consortium (Comprehensive Resistance Prediction for Tuberculosis: an International Consortium) to enable the measurement of MICs of 14 different antituberculosis (anti-TB) compounds for >30,000 clinical Mycobacterium tuberculosis isolates. Unlike the MYCOTB plate, on which the UKMYC5 plate is...

    Paola M. V. Rancoita, Federica Cugnata, Ana LuĂ­za Gibertoni Cruz, Emanuele Borroni, Sarah J. Hoosdally, Timothy M. Walker, Clara Grazian, Timothy J. Davies, Timothy E. A. Peto, Derrick W. Crook, Philip W. Fowler, Daniela M. Cirillo, for the CRyPTIC Consortium
  • Clinical Therapeutics
    Determining the Clinical Utility of an Absolute Procalcitonin Value for Predicting a Positive Culture Result
    Erica M. Caffarini, Joshua DeMott, Gourang Patel, Ishaq Lat
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